Our Ingredients
Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40
Salmon extract DNA
Tranexamic acid
High-purity 3MDa hyaluronic acid, Trehalose & Glutathion
ABOUT BOTULINUM
TEST RESULTS
SAFETY TESTS
REGISRTAION STATUS
Botulinum
The name ‘botulinum’ comes from the Latin word ‘Botulus’, which means black sausage.’Botulinum toxin’ is a neurotoxin protein produced by the bacterium ‘Clostridium botulinum’ that inhibits the secretion of neurotransmitters. Botulinum toxin’ medicine injection is used to treat neuromuscular disorders using temporary paralysis. It is also widely used commercially for medicines, cosmetics, and research.
Botulinum
The name ‘botulinum’ comes from the Latin word ‘Botulus’, which means black sausage.’Botulinum toxin’ is a neurotoxin protein produced by the bacterium ‘Clostridium botulinum’ that inhibits the secretion of neurotransmitters. Botulinum toxin’ medicine injection is used to treat neuromuscular disorders using temporary paralysis. It is also widely used commercially for medicines, cosmetics, and research.
DermoToxin
In recent years, ‘DermoToxin’, which injects ‘Botulinum toxin’ medicine directly into the dermal layer of the skin rather than the muscle layer, has become a general procedure for skin improvement in a dermatology hospital.
‘Dermotoxin’ treatment is known to have a effects not only to improve natural facial wrinkles but also to hydration, pore improvement, bright skin tone, elasticity and lifting compared to general intramuscular injection. It is known as a disadvantage that the pain from injection procedure due to the large number on sites and relatively short duration of efficacy.
DermooToxin
In recent years, ‘DermooToxin’, which injects ‘Botulinum toxin’ medicine directly into the dermal layer of the skin rather than the muscle layer, has become a general procedure for skin improvement in a dermatology hospital.
‘Dermotoxin’ treatment is known to have a effects not only to improve natural facial wrinkles but also to hydration, pore improvement, bright skin tone, elasticity and lifting compared to general intramuscular injection. It is known as a disadvantage that the pain from injection procedure due to the large number on sites and relatively short duration of efficacy.
OUR INGREDIENT
Structure of ‘Methionyl r-Clostridium
Botulinum Polypeptide-1 Hexapeptide-40′
‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ is developed as recombination of peptide called âMTDâ (Macro-molecule Transmission Domain) that has function of skin and target cell permeation and, active protein of botulinum toxin called ‘Light Chain’.  ‘MTD’ applied to our ingredient is originated and developed from botulinum toxin ‘Heavy Chain’, ,which performing a cell-penatration function of botulinum toxin.Â
OUR INGREDIENT
Structure of ‘Methionyl r-Clostridium
Botulinum Polypeptide-1 Hexapeptide-40′
‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ is developed as recombination of peptide called âMTDâ (Macro-molecule Transmission Domain) that has function of skin and target cell permeation and, active protein of botulinum toxin called ‘Light Chain’.  ‘MTD’ applied to our ingredient is originated and developed from botulinum toxin ‘Heavy Chain’, ,which performing a cell-penatration function of botulinum toxin.Â
Insert     MTD    ~20 amino acid                                                          Name : Clostridium botulinum neurotoxin type A light chain
          Lc      Amino acid: 447 amino acid                                                    Sequence : Clostridium botulinum type A Hall Lc
         Tag      Hexa-histidine
Insert     MTD    ~20 amino acid                                                          Name : Clostridium botulinum neurotoxin type A light chain
          Lc      Amino acid: 447 amino acid                                                    Sequence : Clostridium botulinum type A Hall Lc
         Tag      Hexa-histidine
COMPARISON IF BOTULINUM INGREDIENTS
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Â
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Composition | Composition | Composition |
Heavy chain : Nerve cell penetration | Heavy chain : Nerve cell penetration | Light Chain + MTD |
Commercialization | Commercialization | Commercialization |
1980 | 1995 | 2014 |
Example | Example | Example |
BOTOX (Allergan, US) | XEOMIN (Merz, Germany) | BLOOM Planet LTD |
Molecular weight | Molecular weight | Molecular weight |
900KDa | 150KDa | 50KDa |
Skin And Cell Penetration Technology of MTD
Platform technology to improve transdermal delivery ofÂ
‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’
1st ActionÂ
 Improved penetration of functional materials into stratum corneum and other skin layers by MTD.
2nd Action Â
Maximized efficacy through the delivery of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ into specific targets.
Skin And Cell Penetration Technology of MTD
Platform technology to improve transdermal delivery ofÂ
‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’
1st ActionÂ
 Improved penetration of functional materials into stratum corneum and other skin layers by MTD.
2nd Action Â
Maximized efficacy through the delivery of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ into specific targets.
Modality of Intracellular delivery of MTD in ‘Methionyl
r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40′
Permeate in to cell by MTD technology shows very high Permeability and significantly less cell damage then nano liposome or common peptide ingredients(PTD).
Modality of Intracellular delivery of MTD in ‘Methionyl
r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40′
Permeate in to cell by MTD technology shows very high Permeability and significantly less cell damage then nano liposome or common peptide ingredients(PTD).
MECHANISM ON NERVE CELL
SNARE Complex Exocitosis Acetylcholine DiffusionÂ
Binds to the Receptors on the Muscle
Approaching to the Cell
Moves to the SNAP25Â
Methionyl r-Clostridium BotulinumÂ
Polypeptide-1 Hexapeptide-40
Direct Cell Membrane Translocation
Cleaves SNAP25 by Light Chain Part
TEST RESULTS
Recombinant GST-SNAP25-EGFP is purified in order to review activation of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ protein. Since wild type Botulinum Toxin Light Chain cleaves an C-terminal part of SNAP25, GST & EGFP are recombined with 25kDa size on each N-terminal and C-terminal, and purified in order to authentically confirm cleavage form. 75kDa GST-SNAP25-EGFP is cleaved into 50kDa and 25kDa proteins by ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’.
SNAP25 Cleavage
Size alteration after GST-SNAP25-EGFP cleavage
in-vitro SNAP25 cleavage assay
React GST-SNAP25-EGFR protein with various dose of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ in vitro to see if GST-SNAP25-EGFP is cleaved by ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’. As a result, GST-SNAP25-EGFP protein is cleaved into GST-SNAP25(50kDa) and EGFP(25kDa) depend on dose of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’.
Permeation of keratinocytes
Cell treatment on Keratinocytes cell with FITC conjugated âMethionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40â protein. After 1~6 hours, protein can be seen inside the cell. This result shows that âMethionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40â can penetrate skin.
* Treatment condition
– HaCaT cell
– Peptide : 5Ξm
SAFETY TESTS
The following safety tests were performed on the raw material ” Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40 ”.
   – Cell toxicity (in-House Lab.)
  – Bacterial Reverse Mutation Assay test (GLP Lab.)
  – Chromosomal test (GLP Lab.)
The following additional tests are conducted on the safety of ‘Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40’ and animal testing of this ingredient is never carried out.
  – Skin Irritation using EPISKIN
  – Eye Irritation using EpiOcular testâ
  – Skin Sensitization : Local Lymph node Assay : BrdU-ELISA)â
  – in vitro 3T3 NRU Photoxity TestÂ
CLINICAL TESTS
Effectivenss | Ratio | |
Roughness | 16/22 | 72 % |
Net Elasticity | 22/22 | 100 % |
Nasolabial line | 15/22 | 68 % |
REGISTRAION STATUS
“Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40 ” was registered on the International Cosmetics Ingredient Dictionary in December 2014. This can be found on page 2096 of the ICID 2016 Vol.2. The INCI name “Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40” is not defined by the applicant company. This is the name assigned by the PCPC who organizes ICID in Washington and global cosmetic companies for classification of the ingredient. ‘Clostridium Botulinum’ designation on INCI name proves that the material actually derived from ‘Botulinum Toxin ‘.
However, the name ‘Clostridium Botulinum’ is not only important. Botulinum toxin light chain’ alone can not can not penetrate nerve cells even it penetrated in to the skin, so it can not be effective. The core technology of MTD which realizes infiltration into the skin and infiltration of nerve cells has been applied, and botulinum-derived ingredient that we have dreamed of has been born. The ingredient has name ‘Clostridium Botulinum’ can be developed but the technology and efficacy can not be imitated.
REGISTRAION STATUS
“Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40 ” was registered on the International Cosmetics Ingredient Dictionary in December 2014. This can be found on page 2096 of the ICID 2016 Vol.2. The INCI name “Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40” is not defined by the applicant company. This is the name assigned by the PCPC who organizes ICID in Washington and global cosmetic companies for classification of the ingredient. ‘Clostridium Botulinum’ designation on INCI name proves that the material actually derived from ‘Botulinum Toxin ‘.
However, the name ‘Clostridium Botulinum’ is not only important. Botulinum toxin light chain’ alone can not can not penetrate nerve cells even it penetrated in to the skin, so it can not be effective. The core technology of MTD which realizes infiltration into the skin and infiltration of nerve cells has been applied, and botulinum-derived ingredient that we have dreamed of has been born. The ingredient has name ‘Clostridium Botulinum’ can be developed but the technology and efficacy can not be imitated.
Novel cell penetrating peptide, conjugate thereof with botulinum toxin, and use thereof Patent pending technology patent of ” Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40 ” is currently being filed in eight major countries in the world. This technology patent was developed through joint research between ATGC Co., Ltd., which possesses botulinum toxin technology, and Procel Therapeutics Co., Ltd., which possesses skin and target cell penetration technology.
BPMed Cosmetic Co., Ltd. owns the exclusive right to use the patent and the exclusive right to use the material.
- Completed patent registration in Korea (KR.10-1882461)
- Completed patent registration in Japan (JP.6243577)
- Completed patent registration in Russia (RUS.2670135)
- Completed patent registration in USA (US.10300118B2)
- Completed patent registration in Canada (CA.2,949,653)
- Completed patent registration in China(CN.106459155B)
- Registration in European (EP.3156412)
Novel cell penetrating peptide, conjugate thereof with botulinum toxin, and use thereof Patent pending technology patent of ” Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40 ” is currently being filed in eight major countries in the world. This technology patent was developed through joint research between ATGC Co., Ltd., which possesses botulinum toxin technology, and Procel Therapeutics Co., Ltd., which possesses skin and target cell penetration technology.
BPMed Cosmetic Co., Ltd. owns the exclusive right to use the patent and the exclusive right to use the material.
- Completed patent registration in Korea (KR.10-1882461)
- Completed patent registration in Japan (JP.6243577)
- Completed patent registration in Russia (RUS.2670135)
- Completed patent registration in USA (US.10300118B2)
- Completed patent registration in Canada (CA.2,949,653)
- Completed patent registration in China(CN.106459155B)
- Registration in European (EP.3156412)
2. Salmon extract DNA
BENEFITS AND MECHANISMS:
- Improving microcirculation via process called angiogenesis. PDRN causes increased expression of a growth factor called Vascular Endothelial Growth Factor (VEGF) needed for the growth of blood vessels. Blood vessels are the highway for oxygen and nutrients for cells to grow and repair.
- Anti-inflammatory effects on tissues. Inflammation generates free radicals which damages the cells. If these damaged cells are unable to repair themselves, the cells will be removed via apoptosis, our bodyâs natural repair system. Through multiple mechanisms and cytokines, PDRN has been shown to have an anti-inflammatory effect and even reduce scar formation.
- Improves skin texture and elasticity. Collagen synthesis is enhanced by fibroblast activation. This also results in treatment of fine lines in the skin.
- Tissue repair and wound healing. PDRN salvages DNA and RNA fragments from damaged cells to incorporate them into new DNA for normal cell proliferation and recovery .
- Protection from UV damage- UV rays from the sun cause damage to our DNA and this accelerates signs of aging. PDRN has been shown to enhance DNA repair and clearance of toxic by-products of UV-damaged cells .
WHY SALMON IS SAFE?
The PDRN/DNA is derived from Salmon germ cells (thatâs science speak for Salmon sperm cells).
The PDRN/DNA is then subjected to a very rigorous purification process that removes any antigens or proteins that might cause immune or biocompatibility reactions. Salmon DNA is very similar to human DNA. As alluded to above, the rigorous purification that the salmon cells are subjected to removes any proteins that may cause immune reactions.
3. Tranexamic acid
The basic chemical structure is trans-4 aminomethylcyclohexanecarboxylic acid (trans-AMCHA). It inhibits the plasminogen activator by reversibly blocking the lysine binding sites on both plasminogen and plasmin, a molecule responsible for degradation of fibrin. Plasmin is also known to play a role in activation of complement, neutrophils and monocytes and its inhibition thus may also have an anti-inflammatory effect.
Studies have found that it can inhibit UV- induced melanin synthesis by âblocking the interaction between keratinocytes [aka skin cells] and melanocytes [aka, melanin-producing cells],Â
4. High-purity 3MDa hyaluronic acid, Trehalose & Glutathion
3D HA Medical Grade
An innovative concept obtained through a unique process that enriches products with Hyaluronic Acid molecules with 3 different ranges of molecular weight. Due to their nature, they penetrate different layers of the skin, giving birth to a 3D uniform structure in the skin: shorter chains penetrate deeper than longer ones, building up a homogeneous and effective system to restore the inflamed or aged skins.
âĒ Restoration and optimal hydration.
âĒ Aging prevention.
âĒ Healing properties.
âĒ Natural barrier for the skin.
âĒ Preventing from aggressions and preserving the right nutrients.
âĒ Immediately visible effects.
âĒ Enhancement of skin brightness.
âĒ Improvement in skin texture.
âĒ Improvement in skin elasticity and tone.
Trehalose, Arginine and Glutathion
Enhanced moisturizing by superior water adsorption capacity Enhanced skin elasticity through fibroblast cell activation and antioxidant function through increasing the stability of vitamin C and E
3D HA Medical Grade
An innovative concept obtained through a unique process that enriches products with Hyaluronic Acid molecules with 3 different ranges of molecular weight. Due to their nature, they penetrate different layers of the skin, giving birth to a 3D uniform structure in the skin: shorter chains penetrate deeper than longer ones, building up a homogeneous and effective system to restore the inflamed or aged skins.
âĒ Restoration and optimal hydration.
âĒ Aging prevention.
âĒ Healing properties.
âĒ Natural barrier for the skin.
âĒ Preventing from aggressions and preserving the right nutrients.
âĒ Immediately visible effects.
âĒ Enhancement of skin brightness.
âĒ Improvement in skin texture.
âĒ Improvement in skin elasticity and tone.
Trehalose, Arginine and Glutathion
Enhanced moisturizing by superior water adsorption capacity Enhanced skin elasticity through fibroblast cell activation and antioxidant function through increasing the stability of vitamin C and E
